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Proceedings Paper

Femtosecond laser fabrication of fiber based optofluidic platform for flow cytometry applications
Author(s): Murat Serhatlioglu; Caglar Elbuken; Bulend Ortac; Mehmet E. Solmaz
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Paper Abstract

Miniaturized optofluidic platforms play an important role in bio-analysis, detection and diagnostic applications. The advantages of such miniaturized devices are extremely low sample requirement, low cost development and rapid analysis capabilities. Fused silica is advantageous for optofluidic systems due to properties such as being chemically inert, mechanically stable, and optically transparent to a wide spectrum of light. As a three dimensional manufacturing method, femtosecond laser scanning followed by chemical etching shows great potential to fabricate glass based optofluidic chips. In this study, we demonstrate fabrication of all-fiber based, optofluidic flow cytometer in fused silica glass by femtosecond laser machining. 3D particle focusing was achieved through a straightforward planar chip design with two separately fabricated fused silica glass slides thermally bonded together. Bioparticles in a fluid stream encounter with optical interrogation region specifically designed to allocate 405nm single mode fiber laser source and two multi-mode collection fibers for forward scattering (FSC) and side scattering (SSC) signals detection. Detected signal data collected with oscilloscope and post processed with MATLAB script file. We were able to count number of events over 4000events/sec, and achieve size distribution for 5.95μm monodisperse polystyrene beads using FSC and SSC signals. Our platform shows promise for optical and fluidic miniaturization of flow cytometry systems.

Paper Details

Date Published: 28 February 2017
PDF: 8 pages
Proc. SPIE 10058, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII, 100580I (28 February 2017); doi: 10.1117/12.2252092
Show Author Affiliations
Murat Serhatlioglu, Bilkent Univ. (Turkey)
Caglar Elbuken, Bilkent Univ. (Turkey)
Bulend Ortac, Bilkent Univ. (Turkey)
Mehmet E. Solmaz, Izmir Katip Celebi Univ. (Turkey)

Published in SPIE Proceedings Vol. 10058:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII
Israel Gannot, Editor(s)

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