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Proceedings Paper

Visualization of active ingredients uptake in seed coats with stimulated Raman scattering microscopy
Author(s): Chun-Chin Wang; Fei-Yian Yoong; Steven Penfield; Julian Moger
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Paper Abstract

The tissues surrounding the seeds play an important role in the control of germination vigour and in the uptake of active ingredients (AIs) applied as seed dressings. The seeds can reduce costs through more efficient germination rates and have less environmental impact due to more efficient use of AIs. In this study, we use epi-detected stimulated Raman scattering (SRS) microscopy with a fiber laser source for analyzing modes of D2O into intact seeds and determining uptake kinetics. We demonstrate that SRS microscopy is an ideal tool for visualizing uptake of AIs and worthy of further applications in real field conditions.

Paper Details

Date Published: 21 February 2017
PDF: 5 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006928 (21 February 2017); doi: 10.1117/12.2252080
Show Author Affiliations
Chun-Chin Wang, Univ. of Exeter (United Kingdom)
Fei-Yian Yoong, John Innes Ctr. (United Kingdom)
Steven Penfield, John Innes Ctr. (United Kingdom)
Julian Moger, Univ. of Exeter (United Kingdom)

Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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