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Proceedings Paper

Blue LED induced thermal effects in wound healing: experimental evidence in an in vivo model of superficial abrasions
Author(s): Francesca Rossi; Riccardo Cicchi; Giada Magni; Francesca Tatini; Stefano Bacci; Gaia Paroli; Domenico Alfieri; Cristina Tripodi; Gaetano De Siena; Francesco S. Pavone; Roberto Pini
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Paper Abstract

A faster healing process was observed in superficial skin wounds after irradiation with a blue LED (EmoLED) photocoagulator. EmoLED is a compact handheld device, used to induce a thermal effect and thus coagulation in superficial abrasions. We present the results of an in vivo study, conducted in a mouse model, to analyze the induced wound healing. Two superficial abrasions were produced on the back of the mice: one area was treated with EmoLED (1.4 W/cm2, 30 s treatment time), while the other one was left naturally recovering. During the treatment, a temperature around 40-45°C was induced on the abrasion surface. Mice back healthy skin was used as a control. The animals underwent a follow up study and were sacrificed at 0, 1, 3, 6, 9, 12, 18, 21, 24 hours p.o. and 6 days p.o.. Samples from the two abraded areas were harvested and examined by histopathological and immunofluorescence analysis, SHG imaging and confocal microscopy. The aim of the study was to investigate the inflammatory infiltrate, mastocyte population, macrophage subpopulation, fibroblasts and myofibroblasts. Our results show that soon after the treatment, both the inflammatory infiltrate and the M1 macrophage subpopulation appear earlier in the treated, compared to a delayed appearance in the untreated samples. There was no alteration in collagen morphology in the recovered wound. This study confirms the preliminary results obtained in a previous study on a rat model: the selective photothermal effect we used for inducing immediate coagulation in superficial wounds seems to be associated to a faster and improved healing process.

Paper Details

Date Published: 22 February 2017
PDF: 6 pages
Proc. SPIE 10066, Energy-based Treatment of Tissue and Assessment IX, 100660B (22 February 2017); doi: 10.1117/12.2251947
Show Author Affiliations
Francesca Rossi, Istituto di Fisica Applicata "Nello Carrara" (Italy)
Riccardo Cicchi, Istituto Nazionale di Ottica (Italy)
Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Giada Magni, Istituto di Fisica Applicata "Nello Carrara" (Italy)
Francesca Tatini, Istituto di Fisica Applicata "Nello Carrara" (Italy)
Stefano Bacci, Univ. degli Studi di Firenze (Italy)
Gaia Paroli, Univ. degli Studi di Firenze (Italy)
Domenico Alfieri, Light4Tech Firenze S.r.l. (Italy)
Cristina Tripodi, Light4Tech Firenze S.r.l. (Italy)
Gaetano De Siena, Istituto di Fisica Applicata "Nello Carrara" (Italy)
Francesco S. Pavone, Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Istituto Nazionale di Ottica (Italy)
Univ. degli Studi di Firenze (Italy)
Roberto Pini, Istituto di Fisica Applicata "Nello Carrara" (Italy)

Published in SPIE Proceedings Vol. 10066:
Energy-based Treatment of Tissue and Assessment IX
Thomas P. Ryan, Editor(s)

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