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Proceedings Paper

Multimodal fluorescence and photoacoustic microscopy in the frequency domain
Author(s): Gregor Langer; Bianca Buchegger; Jaroslaw Jacak; Karoline Pfeffer; Thomas A. Klar; Thomas Berer
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Paper Abstract

In this paper a multimodal optical resolution microscope in frequency domain is introduced. Fluorescence and photoacoustic signals are generated simultaneously using an amplitude modulated diode laser. The resulting signals are recorded by a lock-in amplifier. By scanning the sample, two-dimensional plots of the fluorescence and photoacoustic signals can be generated. In this paper, the signal-to-noise ratios of amplitude modulated signals are compared to those expected for pulsed excitation under the assumption that the maximum permissible exposure limits are fulfilled. Furthermore, it is described how to determine the excited state life-times from the measured frequency responses of the fluorescence or photoacoustic signals, respectively.

Paper Details

Date Published: 15 February 2017
PDF: 14 pages
Proc. SPIE 10057, Multimodal Biomedical Imaging XII, 1005707 (15 February 2017); doi: 10.1117/12.2251796
Show Author Affiliations
Gregor Langer, Research Ctr. for Non Destructive Testing GmbH (Austria)
Bianca Buchegger, Johannes Kepler Univ. Linz (Austria)
Jaroslaw Jacak, Johannes Kepler Univ. Linz (Austria)
Univ. of Applied Sciences Upper Austria (Austria)
Karoline Pfeffer, Tierärztliche Praxis Claudia und Michael Grötzschel (Austria)
Thomas A. Klar, Johannes Kepler Univ. Linz (Austria)
Thomas Berer, Research Ctr. for Non Destructive Testing GmbH (Austria)

Published in SPIE Proceedings Vol. 10057:
Multimodal Biomedical Imaging XII
Fred S. Azar; Xavier Intes, Editor(s)

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