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Study on choroidal neovascularization with anti-VEGF treatment in the mouse retina using optical coherence tomography angiography (Conference Presentation)
Author(s): Jang Ryul Park; WooJhon Choi; Jaeryung Kim; Hye Kyong Hong; Yongjoo Kim; Yoonha Hwang; Sang Jun Park; Se Joon Woo; Pilhan Kim; Kyu Hyung Park; Gou Young Koh; Wang-Yuhl Oh
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Paper Abstract

To understand the pathogenesis of ophthalmic disease, utilizing small animal models such as mouse is necessary because of their ease of maintenance and availability. For identifying pathophysiology and drug development of retinal diseases in mouse model, optical coherence tomography angiography (OCTA) is promising imaging modality visualizing not only microstructure but also microvasculature. In this study, we serially imaged 3D structure and angiography of laser-induced choroidal neovascularization (CNV) in the mouse retina with/without anti-VEGF treatment. Also, the volume changes of CNV and avascular region in choroid layer are measured for identifying effects of anti-VEGF. A lab-built high-speed OCTA prototype using the wavelength-swept laser centered at 1040 nm with 230 kHz A-scan rate acquired 3-D volumetric data consisted of 1024 x 1024 x 3 A-scans. The OCTA scanned 1.7 mm x 1.7 mm area around ONH. For obtaining angiography, amplitude decorrelation from 3 consecutive B-scans at each position was generated. Seven days after the laser photocoagulation at mouse retina for generation of the laser-induced CNV, intravitreal administration of Fc and VEGF-Trap was given in the therapeutic arm. The OCTA were performed at 6, 14, 21 and 35 days after laser photocoagulation. Vasculatures of inner retina, outer retina and choroid layers were separately visualized after RPE flattening and layer segmentation. To investigate therapeutic effects of anti-VEGF treatment, the relative area and volume of CNV in outer retina layer is measured. Also, total volume of avascular zone surrounding the laser injury site in choroid layer is also analyzed.

Paper Details

Date Published: 16 May 2017
PDF: 1 pages
Proc. SPIE 10045, Ophthalmic Technologies XXVII, 100450G (16 May 2017); doi: 10.1117/12.2251770
Show Author Affiliations
Jang Ryul Park, KAIST (Korea, Republic of)
WooJhon Choi, KAIST (Korea, Republic of)
Jaeryung Kim, KAIST (Korea, Republic of)
Hye Kyong Hong, Seoul National Univ. Bundang Hospital (Korea, Republic of)
Yongjoo Kim, KAIST (Korea, Republic of)
Yoonha Hwang, KAIST (Korea, Republic of)
Sang Jun Park, Seoul National Univ. Bundang Hospital (Korea, Republic of)
Se Joon Woo, Seoul National Univ. Bundang Hospital (Korea, Republic of)
Pilhan Kim, KAIST (Korea, Republic of)
Kyu Hyung Park, Seoul National Univ. Bundang Hospital (Korea, Republic of)
Gou Young Koh, KAIST (Korea, Republic of)
Wang-Yuhl Oh, KAIST (Korea, Republic of)

Published in SPIE Proceedings Vol. 10045:
Ophthalmic Technologies XXVII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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