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Proceedings Paper

Photoacoustic microscopy of cerebral hemodynamic and oxygen-metabolic responses to anesthetics
Author(s): Rui Cao; Jun Li; Bo Ning; Naidi Sun; Tianxiong Wang; Zhiyi Zuo; Song Hu
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Paper Abstract

General anesthetics are known to have profound effects on cerebral hemodynamics and neuronal activities. However, it remains a challenge to directly assess anesthetics-induced hemodynamic and oxygen-metabolic changes from the true baseline under wakefulness at the microscopic level, due to the lack of an enabling technology for high-resolution functional imaging of the awake mouse brain. To address this challenge, we have developed head-restrained photoacoustic microscopy (PAM), which enables simultaneous imaging of the cerebrovascular anatomy, total concentration and oxygen saturation of hemoglobin (CHb and sO2), and blood flow in awake mice. From these hemodynamic measurements, two important metabolic parameters, oxygen extraction fraction (OEF) and the cerebral metabolic rate of oxygen (CMRO2), can be derived. Side-by-side comparison of the mouse brain under wakefulness and anesthesia revealed multifaceted cerebral responses to isoflurane, a volatile anesthetic widely used in preclinical research and clinical practice. Key observations include elevated cerebral blood flow (CBF) and reduced oxygen extraction and metabolism.

Paper Details

Date Published: 19 April 2017
PDF: 5 pages
Proc. SPIE 10051, Neural Imaging and Sensing, 100510V (19 April 2017); doi: 10.1117/12.2251684
Show Author Affiliations
Rui Cao, Univ. of Virginia (United States)
Jun Li, Univ. of Virginia (United States)
Bo Ning, Univ. of Virginia (United States)
Naidi Sun, Univ. of Virginia (United States)
Tianxiong Wang, Univ. of Virginia (United States)
Zhiyi Zuo, Univ. of Virginia (United States)
Song Hu, Univ. of Virginia (United States)

Published in SPIE Proceedings Vol. 10051:
Neural Imaging and Sensing
Qingming Luo; Jun Ding, Editor(s)

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