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Proceedings Paper

Cell counting system by using single fiber interferometer
Author(s): Seung Seok Lee; Joo Ha Kim; Eun Seo Choi
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Paper Abstract

We proposed a cell-counting method using optical fiber interferometer and demonstrated the performance of the proposed method. The cell counting means the counting or the quantification of individual cells. Its application ranges from the biological research to practical disease diagnosis. As a conventional approach for cell counting, various methods are employed. Among them, flow cytometry is quite accurate and exact method but it uses bulk and expensive optical equipment. When image-based methods are exploited, the limited field of view obtained by microscope is considered for cell counting. From this reason, problem of time consuming for whole cell counting is to be solved. The proposed method utilized single-mode optical fiber and high-speed spectrometer. Light beam having broad spectral bandwidth over 100 nm at 850-nm central wavelength is irradiated to a flow channel through fiber from top to bottom. Different optical path length differences are made whether the cell is passing though the flow channel across the beam area or not. The difference of optical path lengths in the beam area due to the cell induces interference signal depending on optical thickness of the cell. By measuring a series of interferences, the number of cells can be analyzed. The proposed system can be implemented without any expensive and perform the cell counting in the absence of complex image analysis. Interferometer-based cell counting can be a good alternative to the reported cell-counting methods.

Paper Details

Date Published: 28 February 2017
PDF: 6 pages
Proc. SPIE 10058, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII, 1005816 (28 February 2017); doi: 10.1117/12.2251594
Show Author Affiliations
Seung Seok Lee, Chosun Univ. (Korea, Republic of)
Joo Ha Kim, Chosun Univ. (Korea, Republic of)
Eun Seo Choi, Chosun Univ. (Korea, Republic of)

Published in SPIE Proceedings Vol. 10058:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII
Israel Gannot, Editor(s)

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