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Proceedings Paper

Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy
Author(s): D. Gillies; W. Gamal; A. Downes; Y. Reinwald; Y. Yang; A. El Haj; P. O. Bagnaninchi
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Paper Abstract

There is an unmet need in tissue engineering for non-invasive, label-free monitoring of cell mechanical behaviour in their physiological environment. Here, we describe a novel optical coherence phase microscopy (OCPM) set-up which can map relative cell mechanical behaviour in monolayers and 3D systems non-invasively, and in real-time. 3T3 and MCF-7 cells were investigated, with MCF-7 demonstrating an increased response to hydrostatic stimulus indicating MCF-7 being softer than 3T3. Thus, OCPM shows the ability to provide qualitative data on cell mechanical behaviour. Quantitative measurements of 6% agarose beads have been taken with commercial Cell Scale Microsquisher system demonstrating that their mechanical properties are in the same order of magnitude of cells, indicating that this is an appropriate test sample for the novel method described.

Paper Details

Date Published: 21 February 2017
PDF: 8 pages
Proc. SPIE 10067, Optical Elastography and Tissue Biomechanics IV, 100670Y (21 February 2017); doi: 10.1117/12.2251492
Show Author Affiliations
D. Gillies, The Univ. of Edinburgh (United Kingdom)
W. Gamal, The Univ. of Edinburgh (United Kingdom)
A. Downes, The Univ. of Edinburgh (United Kingdom)
Y. Reinwald, Keele Univ. (United Kingdom)
Y. Yang, Keele Univ. (United Kingdom)
A. El Haj, Keele Univ. (United Kingdom)
P. O. Bagnaninchi, The Univ. of Edinburgh (United Kingdom)

Published in SPIE Proceedings Vol. 10067:
Optical Elastography and Tissue Biomechanics IV
Kirill V. Larin; David D. Sampson, Editor(s)

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