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Proceedings Paper

Focus-tunable lens in limited-angle holographic tomography
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Paper Abstract

In this paper a new, hardware-based solution for extending the depth of field in holographic tomography is presented. The solution is based on a 4f system and an electric, focus-tunable lens, which provides fast, motion-free defocusing of the plane conjugate with the camera, which acquires holograms. The optimum parameters for the required axial scanning are provided for a specific model of a commercially available tunable lens. Then, the quality of the system equipped with the designed module is analyzed and the reconstruction of a standard object (microsphere) scanned by the 4f-based defocusing system is presented. Finally, the result of the increased depth of field in the measurement domain is demonstrated with a reconstruction of a mouse fibroblast cell.

Paper Details

Date Published: 17 February 2017
PDF: 9 pages
Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 1007009 (17 February 2017); doi: 10.1117/12.2251462
Show Author Affiliations
A. Kuś, Warsaw Univ. of Technology (Poland)
W. Krauze, Warsaw Univ. of Technology (Poland)
M. Kujawińska, Warsaw Univ. of Technology (Poland)

Published in SPIE Proceedings Vol. 10070:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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