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Proceedings Paper

Simultaneous acquisition of trajectory and fluorescence lifetime of moving single particles
Author(s): Qianqian Wu; Jing Qi; Danying Lin; Wei Yan; Rui Hu; Xiao Peng; Junle Qu
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Paper Abstract

Fluorescence lifetime imaging (FLIM) has been a powerful tool in life science because it can reveal the interactions of an excited fluorescent molecule and its environment. The combination with two-photon excitation (TPE) and timecorrelated single photon counting (TCSPC) provides it the ability of optical sectioning, high time resolution and detection efficiency. In previous work, we have introduced a two-dimensional acousto-optic deflector (AOD) into TCSPC-based FLIM to achieve fast and flexible FLIM. In this work, we combined the AOD-FLIM system with a single particle tracking (SPT) setup and algorithm and developed an SPT-FLIM system. Using the system, we acquired the trajectory and fluorescence lifetime of a moving particle simultaneously and reconstructed a life-time-marked pseudocolored trajectory, which might reflect dynamic interaction between the moving particle and its local environment along its motion trail. The results indicated the potential of the technique for studying the interaction between specific moving biological macromolecules and the ambient micro-environment in live cells.

Paper Details

Date Published: 21 February 2017
PDF: 5 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006922 (21 February 2017); doi: 10.1117/12.2251412
Show Author Affiliations
Qianqian Wu, Shenzhen Univ. (China)
Jing Qi, Shenzhen Univ. (China)
Danying Lin, Shenzhen Univ. (China)
Wei Yan, Shenzhen Univ. (China)
Rui Hu, Shenzhen Univ. (China)
Xiao Peng, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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