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Proceedings Paper

In vivo microscopy of human leucocytes (Conference Presentation)
Author(s): Matan Winer; Daniella Yeheskely-Hayon; Adel Zeidan; Dvir Yelin

Paper Abstract

White blood cells (WBC) analysis is an important part of the complete blood count, providing good indication of the patient’s immune system status. The most common types of WBCs are the neutrophils and lymphocytes that comprise approximately 60% and 30% of the total WBC count, respectively; differentiating between these cells at the point of care would assist in accurate diagnosis of the possible source of infection (viral or bacterial) and in effective prescription of antibiotics. In this work, we demonstrate the potential of spectrally encoded flow cytometry (SEFC) to non-invasively image WBC in human patients, allowing morphology characterization of the main types of WBCs. The optical setup includes a broadband light that was diffracted and focused onto a single transverse line within the cross section of a small blood vessel at the inner patient lip. Light backscattered from the tissue was measured by a high-speed spectrometer, forming a two-dimensional reflectance confocal image of the flowing cells. By imaging at different depths into vessels of different diameters, we determine optimal imaging conditions (i.e. imaging geometry, speed and depth) for counting the total amount of WBCs and for differentiating between their main types. The presented technology could serve for analyzing the immune system status at the point of care, and for studying the morphological and dynamical characteristics of these cells in vivo.

Paper Details

Date Published: 19 April 2017
PDF: 1 pages
Proc. SPIE 10040, Endoscopic Microscopy XII, 100400I (19 April 2017); doi: 10.1117/12.2251403
Show Author Affiliations
Matan Winer, Technion (Israel)
Daniella Yeheskely-Hayon, Technion-Israel Institute of Technology (Israel)
Adel Zeidan, Technion-Israel Institute of Technology (Israel)
Dvir Yelin, Technion-Israel Institute of Technology (Israel)

Published in SPIE Proceedings Vol. 10040:
Endoscopic Microscopy XII
Guillermo J. Tearney M.D.; Thomas D. Wang, Editor(s)

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