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Proceedings Paper

Identification and quantitative evaluation of the fiber structure in the pathological tissue using Mueller matrix microscope
Author(s): Jialing Zhou; Honghui He; Ye Wang; Hui Ma
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Paper Abstract

Fiber structure changes in the various pathological processes, such as the increase of fibrosis in liver diseases, the derangement of fiber in cervical cancer and so on. Currently, clinical pathologic diagnosis is regarded as the golden criterion, but different doctors with discrepancy in knowledge and experience may obtain different conclusions. Up to a point, quantitative evaluation of the fiber structure in the pathological tissue can be of great service to quantitative diagnosis. Mueller matrix measurement is capable of probing comprehensive microstructural information of samples and different wavelength of lights can provide more information. In this paper, we use a Mueller matrix microscope with light sources in six different wavelength. We use unstained, dewaxing liver tissue slices in four stages and the pathological biopsy of the filtration channels from rabbit eyes as samples. We apply the Mueller matrix polar decomposition (MMPD) parameter δ which corresponds to retardance to liver slices. The mean value of abnormal region get bigger when the level of fibrosis get higher and light in short wavelength is more sensitive to the microstructure of fiber. On the other hand, we use the Mueller matrix transformation (MMT) parameter Φ which is associated to the angel of fast axis in the analysis of the slices of the filtration channels from rabbit eyes. The value of kurtosis and the value of skewness shows big difference between new born region and normal region and can reveal the arrangement of fiber. These results indicate that the Mueller matrix microscope has great potential in auxiliary diagnosis.

Paper Details

Date Published: 17 February 2017
PDF: 9 pages
Proc. SPIE 10059, Optical Tomography and Spectroscopy of Tissue XII, 1005926 (17 February 2017); doi: 10.1117/12.2251325
Show Author Affiliations
Jialing Zhou, Tsinghua Univ. (China)
Honghui He, Tsinghua Univ. (China)
Ye Wang, Tsinghua Univ. (China)
Hui Ma, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 10059:
Optical Tomography and Spectroscopy of Tissue XII
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca; Robert R. Alfano, Editor(s)

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