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Proceedings Paper

Spatial Frequency Modulated Imaging (SPIFI) with amplitude or phase grating from a spatial light modulator
Author(s): Michael D. Young; Emerson C. Barbano; Nathan Worts; Jeffrey J. Field; Christopher Hoy; Keith A. Wernsing; Randy A. Bartels; Jeff Squier
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Paper Abstract

Spatial Frequency Modulated Imaging (SPIFI) with single element detection has previously been demonstrated with a time varying amplitude spatial frequency. This has been shown in a variety of modalities (linear, TPEF, SHG) and also with variations on the base design to provide additional dimensions of information. SPIFI is also capable of providing enhanced resolution images. However, the signal-to-noise is a limiting factor in the quality of the resolution enhancement. We present a microscope design which uses a nematic spatial light modulator to provide a time varying amplitude from an amplitude or phase grating. Twophoton excitation fluorescence images of 10-µm fluorescent polystyrene beads are presented using a phase grating. Additionally, the microscope can provide spatial gratings in polarization which provide an alternative means of imaging in third harmonic generation (THG). THG images are provided using an amplitude and polarization-grating modulation pattern.

Paper Details

Date Published: 2 March 2017
PDF: 8 pages
Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692P (2 March 2017); doi: 10.1117/12.2251308
Show Author Affiliations
Michael D. Young, Colorado School of Mines (United States)
Emerson C. Barbano, Univ. de São Paulo (Brazil)
Nathan Worts, Colorado School of Mines (United States)
Jeffrey J. Field, Colorado State Univ. (United States)
Christopher Hoy, Boulder Nonlinear Systems (United States)
Keith A. Wernsing, Colorado State Univ. (United States)
Randy A. Bartels, Colorado State Univ. (United States)
Jeff Squier, Colorado School of Mines (United States)

Published in SPIE Proceedings Vol. 10069:
Multiphoton Microscopy in the Biomedical Sciences XVII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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