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Proceedings Paper

Rapid imaging of mammalian brain slices with a compact light sheet fluorescent microscope
Author(s): Zhengyi Yang; Peter Haslehurst; Suzanne Scott; Nigel Emptage; Kishan Dholakia
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Paper Abstract

Light sheet fluorescent microscopy is able to provide high acquisition speed and high contrast images, as well as the low photo-bleaching and photo-damage brought to the sample. Here we describe a compact setup design optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We report this prototype instrument is capable of rapid imaging wide area of the dendritic or axonal arbor of a dye-filled neuron in hippocampal slice. We also show several applications of this compact light sheet fluorescent microscope, to demonstrate that our approach offers a powerful functionality to the neuroscience community that is not achievable with traditional imaging methods.

Paper Details

Date Published: 8 February 2017
PDF: 7 pages
Proc. SPIE 10051, Neural Imaging and Sensing, 100510T (8 February 2017); doi: 10.1117/12.2251227
Show Author Affiliations
Zhengyi Yang, Univ. of St. Andrews (United Kingdom)
Peter Haslehurst, Univ. of Oxford (United Kingdom)
Suzanne Scott, Univ. of Oxford (United Kingdom)
Nigel Emptage, Univ. of Oxford (United Kingdom)
Kishan Dholakia, Univ. of St. Andrews (United Kingdom)

Published in SPIE Proceedings Vol. 10051:
Neural Imaging and Sensing
Qingming Luo; Jun Ding, Editor(s)

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