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Proceedings Paper

Toward multi-focal spot remote focusing two-photon microscopy for high speed imaging
Author(s): Bei Li; Alexander D. Corbett; Ee Chong; Edward Mann; Tony Wilson; Martin J. Booth; Gil Bub
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Paper Abstract

Optical sectioning techniques using two-photon excitation of fluorescent indicators are central to diverse imaging applications. The limitations of the technique are low speed and undesirable specimen agitation. In our design, high-speed axial scanning is carried out by moving a reference objective to axially displace the focal spot without introducing significant spherical aberration and any agitation of the specimen. Further, the system is configured to allow switching between single spot and multiple focal spot remote focusing to allow either high dynamic range or high speed imaging.

Paper Details

Date Published: 17 February 2017
PDF: 5 pages
Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 1007005 (17 February 2017); doi: 10.1117/12.2251214
Show Author Affiliations
Bei Li, Univ. of Oxford (United Kingdom)
Alexander D. Corbett, Univ. of Exeter (United Kingdom)
Ee Chong, Univ. of Oxford (United Kingdom)
Edward Mann, Univ. of Oxford (United Kingdom)
Tony Wilson, Univ. of Oxford (United Kingdom)
Martin J. Booth, Univ. of Oxford (United Kingdom)
Gil Bub, McGill Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 10070:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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