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Proceedings Paper

Confining Brownian motion of single nanoparticles in an ABELtrap
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Paper Abstract

Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a confocal laser focus. We present an Anti-Brownian ELectrokinetic trap1–7 (pioneered by A. E. Cohen and W. E. Moerner) to hold nanoparticles and individual FoF1-ATP synthase proteins in solution. We are interested in the conformational dynamics of this membrane-bound rotary motor protein that we monitor using single-molecule FRET. The ABELtrap is an active feedback system cancelling the nano-object’s Brownian motion by applying an electric field. We show how the induced electrokinetic forces confine the motion of nanoparticles and proteoliposomes to the centre of the trap.

Paper Details

Date Published: 27 February 2017
PDF: 8 pages
Proc. SPIE 10120, Complex Light and Optical Forces XI, 1012017 (27 February 2017); doi: 10.1117/12.2250550
Show Author Affiliations
Maria Dienerowitz, Friedrich-Schiller-Univ. Jena (Germany)
Thomas Heitkamp, Friedrich-Schiller-Univ. Jena (Germany)
Thomas Gottschall, Friedrich-Schiller-Univ. Jena (Germany)
Abbe Ctr. of Photonics (ACP) Jena (Germany)
Jens Limpert, Friedrich-Schiller-Univ. Jena (Germany)
Abbe Ctr. of Photonics (ACP) Jena (Germany)
Fraunhofer-Institut für Angewandte Optik und Feinmechanik IOF (Germany)
Michael Börsch, Friedrich-Schiller-Univ. Jena (Germany)
Abbe Ctr. of Photonics (ACP) Jena (Germany)

Published in SPIE Proceedings Vol. 10120:
Complex Light and Optical Forces XI
David L. Andrews; Enrique J. Galvez; Jesper Glückstad, Editor(s)

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