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Proceedings Paper

A handheld MEMS-based line-scanned dual-axis confocal microscope for early cancer detection and surgical guidance (Conference Presentation)
Author(s): Ye Chen; Chengbo Yin; Linpeng Wei; Adam K. Glaser; Sanjee Abeytunge; Gary Peterson; Michael J. Mandella; Nader Sanai; Milind Rajadhyaksha; Jonathan T. Liu

Paper Abstract

Considerable efforts have been recently undertaken to develop miniature optical-sectioning microscopes for in vivo microendoscopy and point-of-care pathology. These devices enable in vivo interrogation of disease as a real-time and noninvasive alternative to gold-standard histopathology, and therefore could have a transformative impact for the early detection of cancer as well as for guiding tumor-resection procedures. Regardless of the specific modality, various trade-offs in size, speed, field of view, resolution, contrast, and sensitivity are necessary to optimize a device for a particular application. Here, a miniature MEMS-based line-scanned dual-axis confocal (LS-DAC) microscope, with a 12-mm diameter distal tip, has been developed for point-of-care pathology. The dual-axis architecture has demonstrated superior rejection of out-of-focus and multiply scattered photons compared to a conventional single-axis confocal configuration. The use of line scanning enables fast frame rates (≥15 frames/sec), which mitigates motion artifacts of a handheld device during clinical use. We have developed a method to actively align the illumination and collection beams in this miniature LS-DAC microscope through the use of a pair of rotatable alignment mirrors. Incorporation of a custom objective lens, with a small form factor for in vivo application, enables the device to achieve an axial and lateral resolution of 2.0 and 1.1 microns, respectively. Validation measurements with reflective targets, as well as in vivo and ex vivo images of tissues, demonstrate that this high-speed LS-DAC microscope can achieve high-contrast imaging of fluorescently labeled tissues with sufficient sensitivity for applications such as oral cancer detection and guiding brain-tumor resections.

Paper Details

Date Published: 19 April 2017
PDF: 1 pages
Proc. SPIE 10040, Endoscopic Microscopy XII, 100400W (19 April 2017); doi: 10.1117/12.2250440
Show Author Affiliations
Ye Chen, Univ. of Washington (United States)
Chengbo Yin, Univ. of Washington (United States)
Linpeng Wei, Univ. of Washington (United States)
Adam K. Glaser, Univ. of Washington (United States)
Sanjee Abeytunge, Memorial Sloan-Kettering Cancer Ctr. (United States)
Gary Peterson, Memorial Sloan-Kettering Cancer Ctr. (United States)
Michael J. Mandella, Stanford Univ. School of Medicine (United States)
Nader Sanai, Barrow Neurological Institute (United States)
Milind Rajadhyaksha, Memorial Sloan-Kettering Cancer Ctr. (United States)
Jonathan T. Liu, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 10040:
Endoscopic Microscopy XII
Guillermo J. Tearney M.D.; Thomas D. Wang, Editor(s)

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