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Proceedings Paper

In vivo characterization of structural and optical properties of human skin by combined photothermal radiometry and diffuse reflectance spectroscopy
Author(s): Nina Verdel; Ana Marin; Luka Vidovič; Matija Milanič; Boris Majaron
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Paper Abstract

We have combined two optical techniques to enable simultaneous assessment of structure and composition of human skin in vivo: Pulsed photothermal radiometry (PPTR), which involves measurements of transient dynamics in midinfrared emission from sample surface after exposure to a light pulse, and diffuse reflectance spectroscopy (DRS) in visible part of the spectrum. Namely, while PPTR is highly sensitive to depth distribution of selected absorbers, DRS provides spectral information and thus enables differentiation between various chromophores. The accuracy and robustness of the inverse analysis is thus considerably improved compared to use of either technique on its own. Our analysis approach is simultaneous multi-dimensional fitting of the measured PPTR signals and DRS with predictions from a numerical model of light-tissue interaction (a.k.a. inverse Monte Carlo). By using a three-layer skin model (epidermis, dermis, and subcutis), we obtain a good match between the experimental and modeling data. However, dividing the dermis into two separate layers (i.e., papillary and reticular dermis) helps to bring all assessed parameter values within anatomically and physiologically plausible intervals. Both the quality of the fit and the assessed parameter values depend somewhat on the assumed scattering properties for skin, which vary in literature and likely depend on subject's age and gender, anatomical site, etc. In our preliminary experience, simultaneous fitting of the scattering properties is possible and leads to considerable improvement of the fit. The described approach may thus have a potential for simultaneous determination of absorption and scattering properties of human skin in vivo.

Paper Details

Date Published: 6 February 2017
PDF: 9 pages
Proc. SPIE 10037, Photonics in Dermatology and Plastic Surgery, 100370H (6 February 2017); doi: 10.1117/12.2250161
Show Author Affiliations
Nina Verdel, Jožef Stefan Institute (Slovenia)
Ana Marin, Univ. of Ljubljana (Slovenia)
Luka Vidovič, Jožef Stefan Institute (Slovenia)
Matija Milanič, Jožef Stefan Institute (Slovenia)
Univ. of Ljubljana (Slovenia)
Boris Majaron, Jožef Stefan Institute (Slovenia)
Univ. of Ljubljana (Slovenia)

Published in SPIE Proceedings Vol. 10037:
Photonics in Dermatology and Plastic Surgery
Bernard Choi; Haishan Zeng; Nikiforos Kollias, Editor(s)

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