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Proceedings Paper • Open Access

Multi-scale spectrally resolved quantitative fluorescence imaging system: towards neurosurgical guidance in glioma resection
Author(s): Yijing Xie; Maria Thom; Anna Miserocchi; Andrew W. McEvoy; Adrien Desjardins; Sebastien Ourselin; Tom Vercauteren

Paper Abstract

In glioma resection surgery, the detection of tumour is often guided by using intraoperative fluorescence imaging notably with 5-ALA-PpIX, providing fluorescent contrast between normal brain tissue and the gliomas tissue to achieve improved tumour delineation and prolonged patient survival compared with the conventional white-light guided resection. However, the commercially available fluorescence imaging system relies on surgeon’s eyes to visualise and distinguish the fluorescence signals, which unfortunately makes the resection subjective. In this study, we developed a novel multi-scale spectrally-resolved fluorescence imaging system and a computational model for quantification of PpIX concentration. The system consisted of a wide-field spectrally-resolved quantitative imaging device and a fluorescence endomicroscopic imaging system enabling optical biopsy. Ex vivo animal tissue experiments as well as human tumour sample studies demonstrated that the system was capable of specifically detecting the PpIX fluorescent signal and estimate the true concentration of PpIX in brain specimen.

Paper Details

Date Published: 27 February 2017
PDF: 7 pages
Proc. SPIE 10050, Clinical and Translational Neurophotonics, 1005002 (27 February 2017); doi: 10.1117/12.2250159
Show Author Affiliations
Yijing Xie, Univ. College London (United Kingdom)
Maria Thom, Univ. College London (United Kingdom)
Anna Miserocchi, Uiniv. College London (United Kingdom)
Andrew W. McEvoy, Uiniv. College London (United Kingdom)
Adrien Desjardins, Univ. College London (United Kingdom)
Sebastien Ourselin, Univ. College London (United Kingdom)
Tom Vercauteren, Univ. College London (United Kingdom)

Published in SPIE Proceedings Vol. 10050:
Clinical and Translational Neurophotonics
Steen J. Madsen; Victor X. D. Yang, Editor(s)

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