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Proceedings Paper

Investigation of nanodiamonds interactions in canine blood
Author(s): Michał Wąsowicz; Kulka Marek; Maciej Cićkiewicz; Magdalena Cymerman
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Paper Abstract

The whole blood contains red cells, white cells, and platelets suspended in plasma. In the following study we investigated an impact of nanodiamond particles on blood elements over various periods of time.The material used in the study consisted of samples taken from ten healthy canines (Canis lupus f. domestica) of various age, different blood types and both sexes. The markings were conducted by adding to the blood unmodified diamonds (SND), modified O2 (SO2) suspended in 0,9% NaCl. The blood was put under an impact of two diamond concentrations: 20μl and 100μl. The amount of abnormal cells increased with time. The percentage of echinocytes as a result of interaction with nanodiamonds in various time periods for individual specimens was scarce. In the examined microscopic image a summary was made for 100 white blood cells. Following cells were included in said group: band neutrophils, segmented neutrophils, eosinophils, basophils, lymphocytes, monocytes, lymphocytes with granulates, stimulated lymphocytes, lymphocytes with vacuoles, metamielocytes and smudge cells. The impact of the three diamond types had no clinical importance on red blood cells. After the diamonds mixed with white blood cells, atypical cells came into being, in the range of agranulocytes in stimulated form or with granulates and/or vacuoles. It is supposed that as a result of longlasting exposure a stimulation and vacuolisation takes place, because of the function of the cells.

Paper Details

Date Published: 22 February 2017
PDF: 13 pages
Proc. SPIE 10077, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV, 100771C (22 February 2017); doi: 10.1117/12.2249865
Show Author Affiliations
Michał Wąsowicz, Warsaw Univ. of Life Sciences (Poland)
Kulka Marek, Warsaw Univ. of Life Sciences (Poland)
Maciej Cićkiewicz, Dental Movies (Poland)
Magdalena Cymerman, ALAB Plus Sp. z o.o. (Poland)

Published in SPIE Proceedings Vol. 10077:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV
Alexander N. Cartwright; Dan V. Nicolau; Dror Fixler, Editor(s)

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