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Proceedings Paper

Super-duper chemiluminescent proteins applicable to wide range of bioimaging
Author(s): Kazushi Suzuki; Takeharu Nagai
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Paper Abstract

We report five new spectral variants of bright luminescent protein made by concatenation of the brightest luciferase, NanoLuc, with various color hues of fluorescent proteins. These proteins, which we call enhanced Nano-lanterns (eNLs), allow five-color live-cell imaging without external light illumination as well as detection of single molecules. Furthermore, eNL-based Ca2+ indicators could be used to image long-term Ca2+ dynamics in iPS-derived cardiomyocytes.

Paper Details

Date Published: 8 February 2017
PDF: 3 pages
Proc. SPIE 10052, Optogenetics and Optical Manipulation, 1005202 (8 February 2017); doi: 10.1117/12.2249770
Show Author Affiliations
Kazushi Suzuki, Osaka Univ. (Japan)
Takeharu Nagai, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 10052:
Optogenetics and Optical Manipulation
Samarendra K. Mohanty; Nitish V. Thakor; E. Duco Jansen, Editor(s)

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