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Proceedings Paper

Spheroid imaging of phase-diversity homodyne OCT
Author(s): Naoko Senda; Kentaro Osawa
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Paper Abstract

Non-invasive 3D imaging technique is essential for regenerative tissues evaluation. Optical coherence tomography (OCT) is one of 3D imaging tools with no staining and is used extensively for fundus examination. We have developed Phase-Diversity Homodyne OCT which enables cell imaging because of high resolution, whereas conventional OCT was not used for cell imaging because of low resolution. We demonstrated non-invasive imaging inside living spheroids with Phase-Diversity Homodyne OCT. Spheroids are spheroidal cell aggregates and used as regenerative tissues. Cartilage cells were cultured in low-adhesion 96-well plates and spheroids were manufactured. Cell membrane and cytoplasm of spheroid were imaged with OCT.

Paper Details

Date Published: 16 February 2017
PDF: 4 pages
Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100681I (16 February 2017); doi: 10.1117/12.2249715
Show Author Affiliations
Naoko Senda, Hitachi, Ltd. (Japan)
Kentaro Osawa, Hitachi, Ltd. (Japan)

Published in SPIE Proceedings Vol. 10068:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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