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Proceedings Paper

Development of wide-angle 2D light scattering static cytometry
Author(s): Linyan Xie; Qiao Liu; Changshun Shao; Xuantao Su
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Paper Abstract

We have recently developed a 2D light scattering static cytometer for cellular analysis in a label-free manner, which measures side scatter (SSC) light in the polar angular range from 79 to 101 degrees. Compared with conventional flow cytometry, our cytometric technique requires no fluorescent labeling of the cells, and static cytometry measurements can be performed without flow control. In this paper we present an improved label-free static cytometer that can obtain 2D light scattering patterns in a wider angular range. By illuminating the static microspheres on chip with a scanning optical fiber, wide-angle 2D light scattering patterns of single standard microspheres with a mean diameter of 3.87 μm are obtained. The 2D patterns of 3.87 μm microspheres contain both large-angle forward scatter (FSC) and SSC light in the polar angular range from 40 to 100 degrees, approximately. Experimental 2D patterns of 3.87 μm microspheres are in good agreement with Mie theory simulated ones. The wide-angle light scattering measurements may provide a better resolution for particle analysis as compared with the SSC measurements. Two dimensional light scattering patterns of HL-60 human acute leukemia cells are obtained by using our static cytometer. Compared with SSC 2D light scattering patterns, wide-angle 2D patterns contain richer information of the HL-60 cells. The obtaining of 2D light scattering patterns in a wide angular range could help to enhance the capabilities of our label-free static cytometry for cell analysis.

Paper Details

Date Published: 31 October 2016
PDF: 6 pages
Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100243N (31 October 2016); doi: 10.1117/12.2246168
Show Author Affiliations
Linyan Xie, Shandong Univ. (China)
Qiao Liu, Shandong Univ. (China)
Changshun Shao, Shandong Univ. (China)
Xuantao Su, Shandong Univ. (China)

Published in SPIE Proceedings Vol. 10024:
Optics in Health Care and Biomedical Optics VII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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