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Proceedings Paper

Application study of transport intensity equation in quantitative phase reconstruction
Author(s): Xiaojun Song; Wei Cheng; Chunjuan Wei; Liang Xue; Weijing Liu; Baodan Bai; Fenghong Chu
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Paper Abstract

In order to improve detection speed and accuracy of biological cells, a quantitative non-interference optical phase recovery method is proposed in commercial microscope, taking the red blood cells as the classical phase objects. Three bright field micrographs were collected in the experiment. Utilizing the transport intensity equation (TIE), the quantitative phase distributions of red blood cell are gained and agree well with the previous optical phase models. Analysis shows that the resolution of introduced system reaches sub-micron. This method not only quickly gives quantitative phase distribution of cells, but also measures a large number of cells simultaneously. So it is potential in the use of real-time observing and quantitative analyzing of cells in vivo.

Paper Details

Date Published: 31 October 2016
PDF: 6 pages
Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100243F (31 October 2016); doi: 10.1117/12.2246139
Show Author Affiliations
Xiaojun Song, Shanghai Univ. of Electric Power (China)
Wei Cheng, Shanghai Univ. of Electric Power (China)
Chunjuan Wei, Shanghai Univ. of Electric Power (China)
Liang Xue, Shanghai Univ. of Electric Power (China)
Weijing Liu, Shanghai Univ. of Electric Power (China)
Baodan Bai, Shanghai Univ. of Medicine and Health Sciences (China)
Fenghong Chu, Shanghai Univ. of Electric Power (China)

Published in SPIE Proceedings Vol. 10024:
Optics in Health Care and Biomedical Optics VII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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