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Measuring the biomechanical properties of the actin in MCF-7 breast cancer cell with a combined system of AFM and SIM
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Paper Abstract

Biomechanics of cell plays an important role in the behavior and development of diseases, which has a profound influence on the health, structural integrity, and function of cells. In this study, we proposed a method to assess the biomechanical properties in single breast cancer cell line MCF-7 by combining structured illumination microscopy (SIM) with atomic force microscopy (AFM). High resolution optical image of actin in MCF-7 cell and its elastography were obtained. The result shows that the quantitative resolution was improved by SIM, with 490 nm of conventional fluorescence image and 285 nm of reconstructed SIM image, which could give a precise location for AFM measurement. The elasticity of actin is about in the range of 10~1000 kPa. The proposed methods will be helpful in the understanding and clinical diagnosis of diseases at single cell level.

Paper Details

Date Published: 31 October 2016
PDF: 7 pages
Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002431 (31 October 2016); doi: 10.1117/12.2246021
Show Author Affiliations
Minghai You, Fujian Normal Univ. (China)
Jianling Chen, Fujian Normal Univ. (China)
Yuhua Wang, Fujian Normal Univ. (China)
Ningcheng Jiang, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)
Hongqin Yang, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 10024:
Optics in Health Care and Biomedical Optics VII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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