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Proceedings Paper

Monitoring the elasticity changes of HeLa cells during mitosis by atomic force microscopy
Author(s): Ningcheng Jiang; Yuhua Wang; Jinshu Zeng; Xuemei Ding; Shusen Xie; Hongqin Yang
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Paper Abstract

Cell mitosis plays a crucial role in cell life activity, which is one of the important phases in cell division cycle. During the mitosis, the cytoskeleton micro-structure of the cell changed and the biomechanical properties of the cell may vary depending upon different mitosis stages. In this study, the elasticity property of HeLa cells during mitosis was monitored by atomic force microscopy. Also, the actin filaments in different mitosis stages of the cells were observed by confocal imaging. Our results show that the cell in anaphase is stiffer than that in metaphase and telophase. Furthermore, lots of actin filaments gathered in cells’ center area in anaphase, which contributes to the rigidity of the cell in this phase. Our findings demonstrate that the nano-biomechanics of living cells could provide a new index for characterizing cell physiological states.

Paper Details

Date Published: 31 October 2016
PDF: 6 pages
Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002427 (31 October 2016); doi: 10.1117/12.2245537
Show Author Affiliations
Ningcheng Jiang, Fujian Normal Univ. (China)
Yuhua Wang, Fujian Normal Univ. (China)
Jinshu Zeng, Fujian Medical Univ. (China)
Xuemei Ding, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)
Hongqin Yang, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 10024:
Optics in Health Care and Biomedical Optics VII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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