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Proceedings Paper

Enzyme catalyzed optofluidic biolaser for sensitive ion concentration detection
Author(s): Chaoyang Gong; Yuan Gong; Maung Kyaw Khaing Oo; Yu Wu; Yunjiang Rao; Xudong Fan
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Paper Abstract

The enzyme horseradish peroxidase (HRP) has been extensively used in biochemistry for its ability to amplify a weak signal. By using HRP catalyzed substrate as the gain medium, we demonstrate sensitive ion concentration detection based on the optofluidic laser. The enzyme catalyzed reaction occurs in bulk solution inside a Fabry-Perot laser cavity, where the colorless, non-fluorescent 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) substrate is oxidized to produce highly fluorescent resorufin. Laser emission is achieved when pumped with the second harmonic wave of a Q-switched YAG laser. Further, we use sulfide anion (S2-) as an example to investigate the sensing performance of enzyme catalyzed optofluidic laser. The laser onset time difference between the sample to be tested and the reference is set to be the sensing output. Thanks to the amplification effects of both the enzymatic reaction and laser emission, we achieve a detection limit of 10 nM and a dynamic range of 3 orders of magnitude.

Paper Details

Date Published: 9 December 2016
PDF: 6 pages
Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100131S (9 December 2016); doi: 10.1117/12.2244668
Show Author Affiliations
Chaoyang Gong, Univ. of Electronic Science and Technology of China (China)
Yuan Gong, Univ. of Electronic Science and Technology of China (China)
Univ. of Michigan (United States)
Maung Kyaw Khaing Oo, Univ. of Michigan (United States)
Yu Wu, Univ. of Electronic Science and Technology of China (China)
Yunjiang Rao, Univ. of Electronic Science and Technology of China (China)
Xudong Fan, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 10013:
SPIE BioPhotonics Australasia
Mark R. Hutchinson; Ewa M. Goldys, Editor(s)

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