Share Email Print

Proceedings Paper

Discrimination of three typical amino acids using PARAFAC
Author(s): Hui Yang; Xue Xiao; Xuesong Zhao; Yan Wu; Junjun Zong; Jiesong Ye
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

The intrinsic fluorescence characteristics of tryptophan, tyrosine and phenyloalanine were presented in this paper. The excitation/emission wavelength of tryptophan, tyrosine and phenyloalanine locate at λex/λem=280/350nm, 275nm/303 and 260/280nm respectively. The excitation and emission bands of these bio-fluorophores are quite overlapped within the EEM area whose excitation wavelength/emission wavelength scope is 230-270nm/260-340nm. Using the PARAFAC algorithm, 10 tryptophan, tyrosine and phenyloalanine mixed solutions and three compound amino acids samples were successfully decomposed, the emission profiles, excitation profiles, central wavelengths and the concentration of the three components were retrieved with high precision, and finally, the tyrosine and taurine were detected from 1st and 2nd sample respectively.

Paper Details

Date Published: 19 October 2016
PDF: 7 pages
Proc. SPIE 10155, Optical Measurement Technology and Instrumentation, 1015509 (19 October 2016); doi: 10.1117/12.2243750
Show Author Affiliations
Hui Yang, New Star Application Technology Institute (China)
Xue Xiao, AIOFM (China)
Xuesong Zhao, AIOFM (China)
Yan Wu, New Star Application Technology Institute (China)
Junjun Zong, New Star Application Technology Institute (China)
Jiesong Ye, New Star Application Technology Institute (China)

Published in SPIE Proceedings Vol. 10155:
Optical Measurement Technology and Instrumentation
Sen Han; JiuBin Tan, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?