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Proceedings Paper

Raman imaging of biofilms using gold sputtered fiber optic probes
Author(s): Christina Grace Charlet Christopher; Hariharan Manoharan; Aryasomayajula Subrahmanyam; V. V. Raghavendra Sai
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Paper Abstract

In this work we report characterization of bacterial biofilm using gold sputtered optical fiber probe as substrates for confocal Raman spectroscopy measurements. The chemical composition and the heterogeneity of biofilms in the extracellular polymeric substances (EPS) was evaluated. The spatial distribution of bacterial biofilm on the substrates during their growth phase was studied using Raman imaging. Further, the influence of substrate’s surface on bacterial adhesion was investigated by studying growth of biofilms on surfaces with hydrophilic and hydrophobic coatings. This study validates the use of gold sputtered optical fiber probes as SERS substrates in confocal microscopic configuration to identify and characterize clinically relevant biofilms.

Paper Details

Date Published: 9 December 2016
PDF: 5 pages
Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100130D (9 December 2016); doi: 10.1117/12.2242973
Show Author Affiliations
Christina Grace Charlet Christopher, Indian Institute of Technology Madras (India)
Hariharan Manoharan, Indian Institute of Technology Madras (India)
Aryasomayajula Subrahmanyam, Indian Institute of Technology Madras (India)
V. V. Raghavendra Sai, Indian Institute of Technology Madras (India)

Published in SPIE Proceedings Vol. 10013:
SPIE BioPhotonics Australasia
Mark R. Hutchinson; Ewa M. Goldys, Editor(s)

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