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Proceedings Paper

Quantitative optical imaging of paracetamol-induced metabolism changes in the liver
Author(s): Xiaowen Liang; Haolu Wang; Xin Liu; Michael Roberts
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Paper Abstract

Paracetamol is the most readily available and widely used painkiller. However, its toxicity remains the most common cause of liver injury. The toxicity of paracetamol has been attributing to its toxic metabolite, which depletes cellular glutathione (GSH) stores and reacts within cells to increase oxidative stress, leading to mitochondrial dysfunction and cell necrosis. Multiphoton microscopy (MPM) and fluorescence lifetime imaging (FLIM) can provide quantitative imaging of biological tissues and organs in vivo and allow direct visualization of cellular events, which were used to monitor cellular metabolism in paracetamol-induced toxicity in this study. To better understand mechanisms of paracetamol induced liver injury, the redox ratio of NADH/FAD in liver cells were detected and quantified by MPM imaging to represent the relative rates of glycolysis and oxidative phosphorylation within cells. Compared to normal liver, average fluorescence lifetime of NADH and redox ratio of NADH/FAD in hepatocytes was significantly decreased after paracetamol overdose for 12 and 24 hrs, reflecting impaired metabolic activity. GSH levels of treatment groups were significantly lower than those of normal livers, with gradually decreasing from periportal to centrilobular zonation. This imaging technique has significant implications for investigating metabolic mechanisms of paracetamol toxicity.

Paper Details

Date Published: 9 December 2016
PDF: 4 pages
Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100131H (9 December 2016); doi: 10.1117/12.2242962
Show Author Affiliations
Xiaowen Liang, The Univ. of Queensland (Australia)
Haolu Wang, The Univ. of Queensland (Australia)
Xin Liu, The Univ. of Queensland (Australia)
Michael Roberts, The Univ. of Queensland (Australia)
The Univ. of South Australia (Australia)

Published in SPIE Proceedings Vol. 10013:
SPIE BioPhotonics Australasia
Mark R. Hutchinson; Ewa M. Goldys, Editor(s)

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