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Proceedings Paper

Detection of mercury compounds using invertase-glucose oxidase-based biosensor
Author(s): A. Amine; C. Cremisini; Giuseppe Palleschi
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Paper Abstract

Mercury compounds have been determined with an electrochemical biosensor based on invertase inhibition. When invertase is in the presence of mercury its activity decreases; this causes a decrease of glucose production which is monitored by the glucose sensor and correlated to the concentration of mercury in solution. Parameters as pH, enzyme concentration, substrate concentration, and reaction and incubation time were optimized. Mercury compounds determination using soluble or immobilized invertase were reported. Results show that the inhibition was competitive and reversible. Mercury compounds can be detected directly in aqueous solution in the range 2 - 10 ppb.

Paper Details

Date Published: 9 October 1995
PDF: 12 pages
Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); doi: 10.1117/12.224103
Show Author Affiliations
A. Amine, Faculte des Sciences et Techniques (Morocco)
C. Cremisini, ENEA (Italy)
Giuseppe Palleschi, Univ. di Napoli Federico II (Italy)

Published in SPIE Proceedings Vol. 2504:
Environmental Monitoring and Hazardous Waste Site Remediation
Tuan Vo-Dinh, Editor(s)

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