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Proceedings Paper

Investigation of energy transfer between semiconducting polymer dot donors and hydrophilic and hydrophobic Cy5 acceptors
Author(s): Kelsi Lix; W. Russ Algar
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Paper Abstract

Semiconducting polymer dots (Pdots) are rapidly emerging fluorescent probes for bioanalysis. Pdots have extraordinarily strong absorption and bright emission compared to other commonly used fluorescent probes, making them very attractive for applications involving Förster resonance energy transfer (FRET). Here, we investigated two FRET systems with green-emitting poly(9,9-dioctylfluorene-alt-benzothiadiazole) (F8BT) Pdots as donors and two different Cyanine 5 (Cy5) dyes as acceptors. A hydrophilic sulfo-Cy5 dye was directly conjugated to the Pdot surface using carbodiimide chemistry, and a hydrophobic Cy5 dye was observed to spontaneously partition into the core of the Pdot. FRET was observed to depend on the acceptor dye concentration with both systems, and was characterized using a combination of fluorescence emission spectra, excitation spectra, and lifetime measurements. Much stronger quenching of Pdot emission and FRET-sensitized acceptor dye emission were observed for the hydrophobic Cy5 system, and these trends were attributed to reduced donor-acceptor distances in comparison to the hydrophilic sulfo-Cy5 system. Current limitations in the experimental format are discussed. The results show that Pdots are effective FRET donors for acceptor dyes located both within and at the surface of Pdots.

Paper Details

Date Published: 27 September 2016
PDF: 10 pages
Proc. SPIE 9930, Biosensing and Nanomedicine IX, 993007 (27 September 2016); doi: 10.1117/12.2237166
Show Author Affiliations
Kelsi Lix, The Univ. of British Columbia (Canada)
W. Russ Algar, The Univ. of British Columbia (Canada)

Published in SPIE Proceedings Vol. 9930:
Biosensing and Nanomedicine IX
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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