Share Email Print

Proceedings Paper

Computational illumination for 3D phase microscopy (Conference Presentation)
Author(s): Laura Waller

Paper Abstract

This talk will describe new computational microscopy methods for high pixel-count 3D images. We describe two setups employing illumination-side and detection-side aperture coding of angle (Fourier) space for capturing 4D phase-space (e.g. light field) datasets with fast acquisition times. Using a multi-slice forward model and compressive sensing inspired algorithms, we develop efficient 3D reconstruction methods for both incoherent and coherent imaging models, with robustness to scattering and aberrations. Experimentally, we achieve real-time 3D intensity and phase capture with high resolution across a large volume. Such computational approaches to optical microscopy add significant new capabilities to commercial microscopes without significant hardware modification. We will discuss applications in 3D microscopy and all-optical neural activity tracking.

Paper Details

Date Published: 4 November 2016
PDF: 1 pages
Proc. SPIE 9956, Ultrafast Nonlinear Imaging and Spectroscopy IV, 995610 (4 November 2016); doi: 10.1117/12.2235984
Show Author Affiliations
Laura Waller, Univ. of California, Berkeley (United States)

Published in SPIE Proceedings Vol. 9956:
Ultrafast Nonlinear Imaging and Spectroscopy IV
Zhiwen Liu, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?