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Proceedings Paper

A wide field fluorescence lifetime imaging system using a light sheet microscope
Author(s): Phil M. Birch; Lamar Moore; Xiaofei Li; Roger Phillips; Rupert Young; Chris Chatwin
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Paper Abstract

Fluorescence lifetime imaging microscopy (FLIM) has allowed scientists to discern information about the chemical properties of biological processes and has become a vital tool in the life sciences and medical research communities. Measuring the spatial lifetime distribution of the fluorophores as well as the intensity distribution enables users to discern vital information about the chemical environment. It however, remains challenging and often involves slow scanning. We present a new microscope system based on light sheet illumination that uses a micro channel plate (MCP) device called a Capacitive Division Imaging Readout (CDIR) which has been developed by Photek Ltd. The device uses an array of capacitors to move the charge site from the MCP to four pre-amplifiers and time-over-threshold discriminators. This camera has the ability to image photons as well as measure the arrival time, enabling high speed FLIM imaging of biological samples.

Paper Details

Date Published: 27 April 2016
PDF: 8 pages
Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98871O (27 April 2016); doi: 10.1117/12.2230908
Show Author Affiliations
Phil M. Birch, Univ. of Sussex (United Kingdom)
Lamar Moore, Univ. of Sussex (United Kingdom)
Xiaofei Li, Univ. of Sussex (United Kingdom)
Roger Phillips, Univ. of Sussex (United Kingdom)
Rupert Young, Univ. of Sussex (United Kingdom)
Chris Chatwin, Univ. of Sussex (United Kingdom)

Published in SPIE Proceedings Vol. 9887:
Biophotonics: Photonic Solutions for Better Health Care V
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone, Editor(s)

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