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Proceedings Paper

Investigation of magnetic microdiscs for bacterial pathogen detection
Author(s): Keisha Y. Castillo-Torres; Nicolas Garraud; David P. Arnold; Eric S. McLamore
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Paper Abstract

Despite strict regulations to control the presence of human pathogens in our food supply, recent foodborne outbreaks have heightened public concern about food safety and created urgency to improve methods for pathogen detection. Herein we explore a potentially portable, low-cost system that uses magnetic microdiscs for the detection of bacterial pathogens in liquid samples. The system operates by optically measuring the rotational dynamics of suspended magnetic microdiscs functionalized with pathogen-binding aptamers. The soft ferromagnetic (Ni80Fe20) microdiscs exhibit a closed magnetic spin arrangement (i.e. spin vortex) with zero magnetic stray field, leading to no disc agglomeration when in free suspension. With very high surface area for functionalization and volumes 10,000x larger than commonly used superparamagnetic nanoparticles, these 1.5-μm-diameter microdiscs are well suited for tagging, trapping, actuating, or interrogating bacterial targets. This work reports a wafer-level microfabrication process for fabrication of 600 million magnetic microdiscs per substrate and measurement of their rotational dynamics response. Additionally, the biofunctionalization of the microdiscs with DNA aptamers, subsequent binding to E. coli bacteria, and their magnetic manipulation is reported.

Paper Details

Date Published: 13 May 2016
PDF: 8 pages
Proc. SPIE 9863, Smart Biomedical and Physiological Sensor Technology XIII, 98630G (13 May 2016); doi: 10.1117/12.2228610
Show Author Affiliations
Keisha Y. Castillo-Torres, Univ. of Florida (United States)
Nicolas Garraud, Univ. of Florida (United States)
David P. Arnold, Univ. of Florida (United States)
Eric S. McLamore, Univ. of Florida (United States)

Published in SPIE Proceedings Vol. 9863:
Smart Biomedical and Physiological Sensor Technology XIII
Brian M. Cullum; Douglas Kiehl; Eric S. McLamore, Editor(s)

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