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Proceedings Paper

Adaptive optics for confocal laser scanning microscopy with adjustable pinhole
Author(s): Han Woong Yoo; Martin E. van Royen; Wiggert A. van Cappellen; Adriaan B. Houtsmuller; Michel Verhaegen; Georg Schitter
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Paper Abstract

The pinhole plays an important role in confocal laser scanning microscopy (CLSM) for adaptive optics (AO) as well as in imaging, where the size of the pinhole denotes a trade-off between out-of-focus rejection and wavefront distortion. This contribution proposes an AO system for a commercial CLSM with an adjustable square pinhole to cope with such a trade-off. The proposed adjustable pinhole enables to calibrate the AO system and to evaluate the imaging performance. Experimental results with fluorescence beads on the coverslip and at a depth of 40 μm in the human hepatocellular carcinoma cell spheroid demonstrate that the proposed AO system can improve the image quality by the proposed calibration method. The proposed pinhole intensity ratio also indicates the image improvement by the AO correction in intensity as well as resolution.

Paper Details

Date Published: 27 April 2016
PDF: 11 pages
Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 988739 (27 April 2016); doi: 10.1117/12.2227775
Show Author Affiliations
Han Woong Yoo, Vienna Univ. of Technology (Austria)
Delft Univ. of Technology (Netherlands)
Martin E. van Royen, Erasmus Optical Imaging Ctr. (Netherlands)
Wiggert A. van Cappellen, Erasmus Optical Imaging Ctr. (Netherlands)
Adriaan B. Houtsmuller, Erasmus Optical Imaging Ctr. (Netherlands)
Michel Verhaegen, Delft Univ. of Technology (Netherlands)
Georg Schitter, Vienna Univ. of Technology (Austria)

Published in SPIE Proceedings Vol. 9887:
Biophotonics: Photonic Solutions for Better Health Care V
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone, Editor(s)

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