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Proceedings Paper

Biosensors based on Si3N4 asymmetric Mach-Zehnder interferometers
Author(s): Tatevik Chalyan; Laura Pasquardini; Floris Falke; Manuela Zanetti; Romain Guider; Davide Gandolfi; Eric Schreuder; Cecilia Pederzolli; René G. Heideman; Lorenzo Pavesi
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Paper Abstract

In this work, we present a study on photonic biosensors based on Si3N4 asymmetric Mach-Zehnder Interferometers (aMZI) for Aflatoxin M1 (AFM1) detection. AFM1 is an hepatotoxic and a carcinogenic toxin present in milk. The biosensor is based on an array of four Si3N4 aMZI that are optimized for 850nm wavelength. We measure the bulk Sensitivity (S) and the Limit of Detection (LOD) of our devices. In the array, three devices are exposed and have very similar sensitivities. The fourth aMZI, which is covered by SiO2, is used as an internal reference for laser (a VCSEL) and temperature fluctuations. We measured a phase sensitivity of 14300±400 rad/RIU. To characterize the LOD of the sensors, we measure the uncertainty of the experimental readout system. From the measurements on three aMZI, we observe the same value of LOD, which is ≈ 4.5×10−7 RIU. After the sensor characterization on homogeneous sensing, we test the surface sensing performances by flowing specific Aflatoxin M1 and non-specific Ochratoxin in 50 mM MES pH 6.6 buffer on the top of the sensors functionalized with Antigen-Recognising Fragments (Fab’). The difference between specific and non-specific signals shows the specificity of our sensors. A moderate regeneration of the sensors is obtained by using glycine solution.

Paper Details

Date Published: 29 April 2016
PDF: 9 pages
Proc. SPIE 9899, Optical Sensing and Detection IV, 98991S (29 April 2016); doi: 10.1117/12.2227695
Show Author Affiliations
Tatevik Chalyan, Univ. degli Studi di Trento (Italy)
Laura Pasquardini, Fondazione Bruno Kessler (Italy)
Floris Falke, LioniX BV (Netherlands)
Manuela Zanetti, Fondazione Bruno Kessler (Italy)
Romain Guider, Univ. degli Studi di Trento (Italy)
Davide Gandolfi, Univ. degli Studi di Trento (Italy)
Eric Schreuder, LioniX BV (Netherlands)
Cecilia Pederzolli, Fondazione Bruno Kessler (Italy)
René G. Heideman, LioniX BV (Netherlands)
Lorenzo Pavesi, Univ. degli Studi di Trento (Italy)

Published in SPIE Proceedings Vol. 9899:
Optical Sensing and Detection IV
Francis Berghmans; Anna G. Mignani, Editor(s)

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