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Proceedings Paper

Shifted excitation Raman difference spectroscopy for authentication of cheese and cheese analogues
Author(s): Kay Sowoidnich; Heinz-Detlef Kronfeldt
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Paper Abstract

Food authentication and the detection of adulterated products are recent major issues in the food industry as these topics are of global importance for quality control and food safety. To effectively address this challenge requires fast, reliable and non-destructive analytical techniques. Shifted Excitation Raman Difference Spectroscopy (SERDS) is well suited for identification purposes as it combines the chemically specific information obtained by Raman spectroscopy with the ability for efficient fluorescence rejection. The two slightly shifted excitation wavelengths necessary for SERDS are realized by specially designed microsystem diode lasers. At 671 nm the laser (optical power: 50 mW, spectral shift: 0.7 nm) is based on an external cavity configuration whereas an emission at 783 nm (optical power: 110 mW, spectral shift: 0.5 nm) is achieved by a distributed feedback laser. To investigate the feasibility of SERDS for rapid and nondestructive authentication purposes four types of cheese and three different cheese analogues were selected. Each sample was probed at 8 different positions using integration times of 3-10 seconds and 10 spectra were recorded at each spot. Principal components analysis was applied to the SERDS spectra revealing variations in fat and protein signals as primary distinction criterion between cheese and cheese analogues for both excitation wavelengths. Furthermore, to some extent, minor compositional differences could be identified to discriminate between individual species of cheese and cheese analogues. These findings highlight the potential of SERDS for rapid food authentication potentially paving the way for future applications of portable SERDS systems for non-invasive in situ analysis.

Paper Details

Date Published: 27 April 2016
PDF: 8 pages
Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98871Z (27 April 2016); doi: 10.1117/12.2227391
Show Author Affiliations
Kay Sowoidnich, Technische Univ. Berlin (Germany)
STFC Rutherford Appleton Lab. (United Kingdom)
Heinz-Detlef Kronfeldt, Technische Univ. Berlin (Germany)

Published in SPIE Proceedings Vol. 9887:
Biophotonics: Photonic Solutions for Better Health Care V
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone, Editor(s)

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