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Proceedings Paper • Open Access

Demonstration of plant fluorescence by imaging technique and Intelligent FluoroSensor
Author(s): Sándor Lenk; Patrik Gádoros; László Kocsányi; Attila Barócsi

Paper Abstract

Photosynthesis is a process that converts carbon-dioxide into organic compounds, especially into sugars, using the energy of sunlight. The absorbed light energy is used mainly for photosynthesis initiated at the reaction centers of chlorophyll-protein complexes, but part of it is lost as heat and chlorophyll fluorescence. Therefore, the measurement of the latter can be used to estimate the photosynthetic activity. The basic method, when illuminating intact leaves with strong light after a dark adaptation of at least 20 minutes resulting in a transient change of fluorescence emission of the fluorophore chlorophyll-a called ‘Kautsky effect’, is demonstrated by an imaging setup. The experimental kit includes a high radiant blue LED and a CCD camera (or a human eye) equipped with a red transmittance filter to detect the changing fluorescence radiation. However, for the measurement of several fluorescence parameters, describing the plant physiological processes in detail, the variation of several excitation light sources and an adequate detection method are needed. Several fluorescence induction protocols (e.g. traditional Kautsky, pulse amplitude modulated and excitation kinetic), are realized in the Intelligent FluoroSensor instrument. Using it, students are able to measure different plant fluorescence induction curves, quantitatively determine characteristic parameters and qualitatively interpret the measured signals.

Paper Details

Date Published: 8 October 2015
PDF: 5 pages
Proc. SPIE 9793, Education and Training in Optics and Photonics: ETOP 2015, 97931T (8 October 2015); doi: 10.1117/12.2223179
Show Author Affiliations
Sándor Lenk, Budapest Univ. of Technology and Economics (Hungary)
Patrik Gádoros, Budapest Univ. of Technology and Economics (Hungary)
László Kocsányi, Budapest Univ. of Technology and Economics (Hungary)
Attila Barócsi, Budapest Univ. of Technology and Economics (Hungary)

Published in SPIE Proceedings Vol. 9793:
Education and Training in Optics and Photonics: ETOP 2015

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