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Proceedings Paper

Effects of low-level laser exposure on calcium channels and intracellular release in cultured astrocytes
Author(s): Thomas S. Mang; Mohammed Mehdi Maneshi; David Wm. Shucard; Susan Hua; Frederick Sachs
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Paper Abstract

Prompted by a study of traumatic brain injury (TBI) in a model system of cultured astrocytes, we discovered that low level laser illumination (LLL) at 660nm elevates the level of intracellular Ca2+. The coherence of the illumination was not essential since incoherent red light also worked. For cells bathed in low Ca2+ saline so that influx was suppressed, the Ca2+ level rose with no significant latency following illumination and consistent with a slow leak of Ca2+ from storage such as from the endoplasmic reticulum and/or mitochondria. When the cells were bathed in normal Ca2+ saline, the internal Ca2+ rose, but with a latency of about 17 seconds from the beginning of illumination. Pharmacologic studies with ryanodine inhibited the light effect. Testing the cells with fluid shear stress as used in the TBI model showed that mechanically induced elevation of cell Ca2+ was unaffected by illumination.

Paper Details

Date Published: 23 March 2016
PDF: 10 pages
Proc. SPIE 9695, Mechanisms of Photobiomodulation Therapy XI, 969509 (23 March 2016); doi: 10.1117/12.2222222
Show Author Affiliations
Thomas S. Mang, Univ. at Buffalo (United States)
Mohammed Mehdi Maneshi, Univ. at Buffalo (United States)
David Wm. Shucard, Univ. at Buffalo (United States)
Susan Hua, Univ. at Buffalo (United States)
Frederick Sachs, Univ. at Buffalo (United States)

Published in SPIE Proceedings Vol. 9695:
Mechanisms of Photobiomodulation Therapy XI
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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