Fluorescent proteins are often used as reporters of protein concentration in biology and biomedicine applications. They can be detected using a fluorimeter equipped with fiber optics for ease of access. However, small changes in the path length due to change in the position, or immersion depth of the optical fiber results in large changes in readings. To alleviate the situation, the fiber is equipped with a fixed-length-extension that provides constant path length. The operation of the fiber equipped fluorimeter is theoretically modelled and practically verified in this paper.

Date Published: 13 May 2016
PDF: 7 pages
Proc. SPIE 9863, Smart Biomedical and Physiological Sensor Technology XIII, 98630J (13 May 2016); doi: 10.1117/12.2221931

Published in SPIE Proceedings Vol. 9863:
Smart Biomedical and Physiological Sensor Technology XIII
Brian M. Cullum; Douglas Kiehl; Eric S. McLamore, Editor(s)