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Proceedings Paper

Two-photon multiplane imaging of neural circuits (Conference Presentation)
Author(s): Weijian Yang; Jae-eun K Miller; Luis Carrillo-Reid; Eftychios Pnevmatikakis; Liam Paninski; Darcy S. Peterka; Rafael Yuste

Paper Abstract

Imaging the neuronal activity throughout the brain with high temporal and spatial resolution is an important step in understanding how the brain works. Two-photon laser scanning microscopy with fluorescent calcium indicators has enabled this type of experiments in vivo. Most of these microscopes acquire images serially, with a single laser beam, limiting the overall imaging speed. To overcome this limit, multiple beamlets can be used to image in parallel multiple regions. Here, we demonstrate a novel scheme of a two-photon laser-scanning microscope that can simultaneously record neuronal activity at multiple planes of the sample with a single photomultiplier tube. A spatial light modulator is used to generate the designated multiple beamlets, and a constrained non-negative matrix factorization algorithm is used to demix the signals from multiple scanned planes. We simultaneously record neuronal activity of multiple layers of a mouse cortex at 10 fps in vivo. This novel imaging scheme provides a powerful tool for mapping the brain activity.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 969016 (26 April 2016); doi: 10.1117/12.2219843
Show Author Affiliations
Weijian Yang, Columbia Univ. (United States)
Jae-eun K Miller, Columbia Univ. (United States)
Luis Carrillo-Reid, Columbia Univ. (United States)
Eftychios Pnevmatikakis, Simons Foundation (United States)
Columbia Univ. (United States)
Liam Paninski, Columbia Univ. (United States)
Darcy S. Peterka, Columbia Univ. (United States)
Rafael Yuste, Columbia Univ. (United States)

Published in SPIE Proceedings Vol. 9690:
Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation
Steen J. Madsen; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Victor X. D. Yang, Editor(s)

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