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Proceedings Paper

Fluorescent nanodiamonds and their use in biomedical research
Author(s): Lorena P. Suarez-Kelly; Isaac V. Rampersaud; Charles E. Moritz; Amanda R. Campbell; Zhiwei Hu; Masfer H. Alkahtani; Fahad S. Alghannam; Phillip Hemmer; William E. Carson III; Arfaan A. Rampersaud
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Paper Abstract

Nanodiamonds containing color-centers produce non-quenching fluorescence that is easily detected. This makes them useful for cellular, proteomic and genomic applications. However, fluorescent nanodiamonds have yet to become popular in the biomedical research community as labeling reagents. We discuss production of nanodiamonds with distinct color-centers and assess their biocompatibility and techniques for bioconjugation. Fluorescent diamonds were fabricated by electron irradiation of high-pressure, high-temperature micron-sized diamonds which generated diamonds with vacancy-related defects (V). These diamonds were annealed to create nitrogen vacancy (NV)-centers then following a milling step were fractionated into nanoparticle sizes of 30, 60, and 95 nm. Optical characterization of Vand NV-center diamonds demonstrated fluorescence in two distinct green and red channels, respectively. In vitro studies demonstrated that these nanodiamonds are biocompatible and readily taken up by murine macrophage cells. Quantification of NV-center nanodiamond uptake by flow cytometry, showed that uptake was independent of nanodiamond size. Confocal microscopy demonstrated that NV-center nanodiamonds accumulate within the cytoplasm of these cells. NV-center nanodiamonds were then conjugated with streptavidin using a short polyethylene chain as linker. Conjugation was confirmed via a catalytic assay employing biotinylated-horseradish peroxidase. We present a technique for large-scale production of biocompatible conjugated V- or NV-center nanodiamonds. Functional testing is essential for standardization of fluorescent nanodiamond bioconjugates and quality control. Large-scale production of bioconjugated fluorescent nanodiamonds is crucial to their development as novel tools for biological and medical applications.

Paper Details

Date Published: 15 March 2016
PDF: 21 pages
Proc. SPIE 9762, Advances in Photonics of Quantum Computing, Memory, and Communication IX, 976205 (15 March 2016); doi: 10.1117/12.2218612
Show Author Affiliations
Lorena P. Suarez-Kelly, The Ohio State Univ. (United States)
Isaac V. Rampersaud, Columbus NanoWorks, Inc. (United States)
Charles E. Moritz, Columbus NanoWorks, Inc. (United States)
Amanda R. Campbell, The Ohio State Univ. (United States)
Zhiwei Hu, The Ohio State Univ. (United States)
Masfer H. Alkahtani, Texas A&M Univ. (United States)
The National Ctr. for Applied Physics, KACST (Saudi Arabia)
Fahad S. Alghannam, Texas A&M Univ. (United States)
The National Ctr. for Applied Physics, KACST (Saudi Arabia)
Phillip Hemmer, Texas A&M Univ. (United States)
William E. Carson III, The Ohio State Univ. (United States)
Arfaan A. Rampersaud, Columbus NanoWorks Inc. (United States)

Published in SPIE Proceedings Vol. 9762:
Advances in Photonics of Quantum Computing, Memory, and Communication IX
Zameer Ul Hasan; Philip R. Hemmer; Hwang Lee; Alan L. Migdall, Editor(s)

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