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Proceedings Paper

Fluorescence intensity enhancement mechanism in presence of plasmonic nanoparticles
Author(s): Sumana Das; Brahmanandam Javvaji; Krishna Harika Villa; Akshata Arikady; Gopalkrishna M. Hegde; D. Roy Mahapatra
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Paper Abstract

Plasmonic nanoparticles have several applications ranging from catalysis to super-resolution imaging and information storage. Maximum density of optical states is confined on the nanoparticle surface, which are collectively excited by electromagnetic wave and are called surface plasmons. Using nanoparticle based plasmonic interaction with biological cells in an optical fiber integrated microfluidic chip, we show enhancement of fluorescence intensity. Signal from in situ imaging is analyzed with various controls to understand the mechanism. The present study is focused on nanoparticle interaction with cells and on optimization strategies to maximize the fluorescence enhancement at the vicinity of the nanoparticles, for important applications such as fluorescence-based biochip platforms. Result is also correlated ZnO nanoparticle effect on fluorescence enhancement, which has different optoelectronic properties compared to gold nanoparticles. Electromagnetic wave field model is employed to simulate the effect of gold and ZnO nanoparticle on cell with the assumption that the nanoparticles are a collection of discrete dipoles, which are ordered with the fluorescence molecules on cell wall. Simulation model shows enhancement of fluorescence intensity is occurred in presence of gold nanoparticles rather than ZnO nanoparticles, which is confirmed with experimental data.

Paper Details

Date Published: 22 April 2016
PDF: 7 pages
Proc. SPIE 9724, Plasmonics in Biology and Medicine XIII, 97240K (22 April 2016); doi: 10.1117/12.2217940
Show Author Affiliations
Sumana Das, Indian Institute of Science (India)
Brahmanandam Javvaji, Indian Institute of Science (India)
Krishna Harika Villa, Indian Institute of Science (India)
Akshata Arikady, Indian Institute of Science (India)
Gopalkrishna M. Hegde, Indian Institute of Science (India)
D. Roy Mahapatra, Indian Institute of Science (India)

Published in SPIE Proceedings Vol. 9724:
Plasmonics in Biology and Medicine XIII
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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