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Proceedings Paper

Oxygen micro-optrodes and their application in aquatic environments
Author(s): Ingo Klimant; Gerhard A. Holst; Michael Kuehl
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Paper Abstract

We present a new fiber-optic oxygen microsensor based on dynamic luminescence quenching which was recently developed for measuring oxygen at high spatial resolution in aquatic sediments and biofilms. Micro-optrodes with a typical tip diameter of 20 to 50 micrometers were fabricated. The fabrication procedure is simple and guarantees a high reproducibility of the calibration curves. The micro-optrodes were characterized with respect to dynamic range, response time, storage, longterm stability, interferences, temperature dependence, photostability, and mechanical stability. A special LED based luminescence intensity measuring instrument was developed. It is battery operated and can be used for field measurements. The micro-optrodes were used to measure oxygen gradients in marine sediments. Comparative measurements were performed with oxgen microelectrodes. The first measurements have shown that oxygen micro-optrodes present a true alternative to existing electrochemical microsensors. Nevertheless, it is obvious, that the measurement of luminescence intensity of the indicator limits their practical application. Therefore a new setup was developed to make oxygen measurements with the luminescence lifetime as parameter.

Paper Details

Date Published: 29 September 1995
PDF: 12 pages
Proc. SPIE 2508, Chemical, Biochemical, and Environmental Fiber Sensors VII, (29 September 1995); doi: 10.1117/12.221753
Show Author Affiliations
Ingo Klimant, Max Planck Institute for Marine Microbiology (Germany)
Gerhard A. Holst, Max Planck Institute for Marine Microbiology (Germany)
Michael Kuehl, Max Planck Institute for Marine Microbiology (Germany)

Published in SPIE Proceedings Vol. 2508:
Chemical, Biochemical, and Environmental Fiber Sensors VII
Annamaria V. Scheggi, Editor(s)

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