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Proceedings Paper

Biomechanical cell analysis using quantitative phase imaging (Conference Presentation)
Author(s): Adam Wax; Han Sang Park; William J. Eldridge

Paper Abstract

Quantitative phase imaging provides nanometer scale sensitivity and has been previously used to study spectral and temporal characteristics of individual cells in vitro, especially red blood cells. Here we extend this work to study the mechanical responses of individual cells due to the influence of external stimuli. Cell stiffness may be characterized by analyzing the inherent thermal fluctuations of cells but by applying external stimuli, additional information can be obtained. The time dependent response of cells due to external shear stress is examined with high speed quantitative phase imaging and found to exhibit characteristics that relate to their stiffness. However, analysis beyond the cellular scale also reveals internal organization of the cell and its modulation due to pathologic processes such as carcinogenesis. Further studies with microfluidic platforms point the way for using this approach in high throughput assays.

Paper Details

Date Published: 27 April 2016
PDF: 1 pages
Proc. SPIE 9719, Biophysics, Biology, and Biophotonics: the Crossroads, 97190H (27 April 2016); doi: 10.1117/12.2217440
Show Author Affiliations
Adam Wax, Duke Univ. (United States)
Han Sang Park, Duke Univ. (United States)
William J. Eldridge, Duke Univ. (United States)

Published in SPIE Proceedings Vol. 9719:
Biophysics, Biology, and Biophotonics: the Crossroads
Adam Wax; Vadim Backman, Editor(s)

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