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Proceedings Paper

Highly sensitive kinesin-microtubule motility assays using SLIM
Author(s): Mikhail Kandel; Kai Wen Teng; Paul R. Selvin; Gabriel Popescu
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Paper Abstract

We provide an experimental demonstration of Spatial Light Interference Microscopy (SLIM) as a tool for measuring the motion of 25 nm tubulin structures without the use of florescence labels. Compared to intensity imaging methods such as phase contrast or DIC, our imaging technique relies on the ratios of images associated with optically introduced phase shifts, thus implicitly removing background illumination. To demonstrate our new found capabilities, we characterize kinesin-based motility continuously over periods of time where fluorescence would typically photobleach. We exploit this new method to compare the motility of microtubules at low ATP concentrations, with and without the tagging proteins formerly required to perform these studies. Our preliminary results show that the tags have a non-negligible effect on the microtubule motility, slowing the process down by more than 10%.

Paper Details

Date Published: 9 March 2016
PDF: 5 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 97180T (9 March 2016); doi: 10.1117/12.2217109
Show Author Affiliations
Mikhail Kandel, Beckman Institute of Advanced Science and Technology (United States)
Univ. of Illinois (United States)
Kai Wen Teng, Univ. of Illinois (United States)
Paul R. Selvin, Univ. of Illinois (United States)
Gabriel Popescu, Beckman Institute for Advanced Science and Technology (United States)
Univ. of Illinois (United States)

Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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