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Proceedings Paper

HoloMonitor M4: holographic imaging cytometer for real-time kinetic label-free live-cell analysis of adherent cells
Author(s): Mikael Sebesta; Peter J. Egelberg; Anders Langberg; Jens-Henrik Lindskov; Kersti Alm; Birgit Janicke
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Paper Abstract

Live-cell imaging enables studying dynamic cellular processes that cannot be visualized in fixed-cell assays. An increasing number of scientists in academia and the pharmaceutical industry are choosing live-cell analysis over or in addition to traditional fixed-cell assays. We have developed a time-lapse label-free imaging cytometer HoloMonitorM4. HoloMonitor M4 assists researchers to overcome inherent disadvantages of fluorescent analysis, specifically effects of chemical labels or genetic modifications which can alter cellular behavior. Additionally, label-free analysis is simple and eliminates the costs associated with staining procedures.

The underlying technology principle is based on digital off-axis holography. While multiple alternatives exist for this type of analysis, we prioritized our developments to achieve the following: a) All-inclusive system – hardware and sophisticated cytometric analysis software; b) Ease of use enabling utilization of instrumentation by expert- and entrylevel researchers alike; c) Validated quantitative assay end-points tracked over time such as optical path length shift, optical volume and multiple derived imaging parameters; d) Reliable digital autofocus; e) Robust long-term operation in the incubator environment; f) High throughput and walk-away capability; and finally g) Data management suitable for single- and multi-user networks.

We provide examples of HoloMonitor applications of label-free cell viability measurements and monitoring of cell cycle phase distribution.

Paper Details

Date Published: 9 March 2016
PDF: 10 pages
Proc. SPIE 9718, Quantitative Phase Imaging II, 971813 (9 March 2016); doi: 10.1117/12.2216731
Show Author Affiliations
Mikael Sebesta, Phase Holographic Imaging AB (Sweden)
Peter J. Egelberg, Phase Holographic Imaging AB (Sweden)
Anders Langberg, Phase Holographic Imaging AB (Sweden)
Jens-Henrik Lindskov, Phase Holographic Imaging AB (Sweden)
Kersti Alm, Phase Holographic Imaging AB (Sweden)
Birgit Janicke, Phase Holographic Imaging AB (Sweden)

Published in SPIE Proceedings Vol. 9718:
Quantitative Phase Imaging II
Gabriel Popescu; YongKeun Park, Editor(s)

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