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Proceedings Paper

Investigation of interfacial peptide-lipid interactions by optical second harmonic generation from tryptophan
Author(s): Beth L. Smiley; Viola Vogel
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Paper Abstract

The adsorption of pentapeptides to lipid monolayers spread at the air-water interface is investigated by optical second harmonic generation (SHG) in the reflection geometry. The nonlinear optical response of the tryptophan side chain present in each of the synthetic peptide sequences chosen was insufficient to allow determination of its molecular orientation within the surface layer at the surface densities obtain by adsorption from the subphase. A difference in the character of peptide adsorption to lipid monolayers of various compositions was observed which depends on the nature of the monolayer interface accessible to the peptides. Results from crude and purified synthetic peptides are compared to point out the impact of potential hydrophobic impurities on the surface properties of the monolayer and on the measured second harmonic signal intensity. SHG studies of peptides and proteins adsorbed to membrane-mimetic lipid and phospholipid monolayers may suggest differences in the chemical nature of the monolayers which impact the ability of peptides and proteins to penetrate and interact with the membrane surface.

Paper Details

Date Published: 25 September 1995
PDF: 8 pages
Proc. SPIE 2547, Laser Techniques for Surface Science II, (25 September 1995); doi: 10.1117/12.221492
Show Author Affiliations
Beth L. Smiley, Univ. of Washington (United States)
Viola Vogel, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 2547:
Laser Techniques for Surface Science II
Janice M. Hicks; Wilson Ho; Hai-Lung Dai, Editor(s)

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