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Proceedings Paper

An integrated optical coherence microscopy imaging and optical stimulation system for optogenetic pacing in Drosophila melanogaster (Conference Presentation)
Author(s): Aneesh Alex; Airong Li; Jing Men; Jason Jerwick; Rudolph E. Tanzi; Chao Zhou; Zhan Zhang; Xianxu Zeng

Paper Abstract

Electrical stimulation is the clinical standard for cardiac pacing. Although highly effective in controlling cardiac rhythm, the invasive nature, non-specificity to cardiac tissues and possible tissue damage limits its applications. Optogenetic pacing of the heart is a promising alternative, which is non-invasive and more specific, has high spatial and temporal precision, and avoids the shortcomings in electrical stimulation. Drosophila melanogaster, which is a powerful model organism with orthologs of nearly 75% of human disease genes, has not been studied for optogenetic pacing in the heart. Here, we developed a non-invasive integrated optical pacing and optical coherence microscopy (OCM) imaging system to control the heart rhythm of Drosophila at different developmental stages using light. The OCM system is capable of providing high imaging speed (130 frames/s) and ultrahigh imaging resolutions (1.5 μm and 3.9 μm for axial and transverse resolutions, respectively). A light-sensitive pacemaker was developed in Drosophila by specifically expressing the light-gated cation channel, channelrhodopsin-2 (ChR2) in transgenic Drosophila heart. We achieved non-invasive and specific optical control of the Drosophila heart rhythm throughout the fly’s life cycle (larva, pupa, and adult) by stimulating the heart with 475 nm pulsed laser light. Heart response to stimulation pulses was monitored non-invasively with OCM. This integrated non-invasive optogenetic control and in vivo imaging technique provides a novel platform for performing research studies in developmental cardiology.

Paper Details

Date Published: 26 April 2016
PDF: 1 pages
Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 96971A (26 April 2016); doi: 10.1117/12.2214671
Show Author Affiliations
Aneesh Alex, Lehigh Univ. (United States)
Airong Li, Massachusetts General Hospital (United States)
Jing Men, Lehigh Univ. (United States)
Jason Jerwick, Lehigh Univ. (United States)
Rudolph E. Tanzi, Massachusetts General Hospital (United States)
Chao Zhou, Lehigh Univ. (United States)
Zhan Zhang, Third Affiliated Hospital of Zhengzhou Univ. (China)
Xianxu Zeng, Lehigh Univ. (United States)

Published in SPIE Proceedings Vol. 9697:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX
Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin, Editor(s)

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